Mosses as bioindicators of air pollution with potentially toxic elements in the Burabay State National Natural Park, Kazakhstan.

The Burabay State National Natural Park is a national park of the great natural and historical values located in the north of Kazakhstan, which has been exposed in recent years to significant anthropogenic impact. The moss biomonitoring was performed in the Borovoye resort community, an important tourist destination in the national park, to identify the level of air pollution. Mosses collected at 29 locations were subjected to neutron activation analysis to determine 36 elements and additionally to ICP-OES to detect the level of Cu and Pb. Factor analysis was applied to check if there are any associations between identified elements and to link them with possible emission sources. According to contamination factor and pollution load indices the investigated area belongs to three classes of pollution: unpolluted, suspected and moderate. Potential ecological risk index calculated for selected elements revealed harmless risk to human health. The level of element obtained in Burabay State National Natural Park was compared with the data available for other national parks.

Assessment of Air Pollution in Ulaanbaatar Using the Moss Bag Technique.

Active moss biomonitoring, the so-called moss bag technique, widely applied in many countries, for the first time, was applied to assess the air quality in Ulaanbaatar (Mongolia). Moss bags with Sphagnum girgensohnii Russow were exposed in triplicate in three different periods: December-February, March-May, and December-May at 13 governmental air quality monitoring stations located in the vicinity of thermal power plants and residential areas. The plant tissue content of Al, Ba, Co, Cd, Cr, Cu, Fe, Mn, P, Pb, Sr, S, V, As, and Zn was determined using inductively coupled plasma-optical emission spectrometry, and a direct mercury analyzer was used to determine the Hg content. The samples in residential areas and near thermal power plants that were exposed for 3 months in winter and for 6 months (winter to spring) were characterized by the highest accumulation of the elements. In the moss bags exposed during spring, maximum accumulation of the determined elements was noted in residential areas and near main roads. Regardless of the exposure time and duration, the highest accumulation of Al, Fe, and V was determined at Dambadarjaa air quality station located near a highway and of Hg near the Amgalan power plant. Significant differences in element accumulation between seasons were observed, thus, the accumulation of Al, Ba, As, Co, Cr, Fe, Pb, V, and Zn was higher in spring, while P and S had higher content in the moss samples exposed during winter. The accumulation of elements over the 6-month exposure period was 1.1-6.7 times higher than that of the 3-month periods. Thus, the 6-month exposure can be considered a reliable deployment period as it ensures an adequate signal in terms of enrichment of pollutants. Factor analysis was applied to highlight the association of elements and to link them with possible sources of emission. Three factors were determined, the first one included Al, As, Ba, Co, Cr, Fe, Mn, Pb, Sr, and V and was identified as a geogenic-anthropogenic, the second (Cu, P, and S) and third (Cd and Zn) factors suggested anthropogenic origin. The Relative accumulation factor and enrichment factor were calculated to evaluate the level of air pollution and possible element sources. Considerable contributors to air pollution were Zn, Fe, As, V, Cr, and Al, which may originate from airborne soil particles of crustal matter or transport, as well as coal combustion for heating and cooking.

Natural and anthropogenic radionuclides concentration with heavy metals analysis of the sediments collected around Novaya Zemlya.

The Dispersal profile of the radioisotopes (226Ra, 232Th, 235U, 40K, 137Cs) along with potentially toxic elements (Cd, Co, Cr, Cu, Ni, Pb, V, Zn, and Hg) in the sediments around the Novaya Zemlya was determined. The task was fulfilled with the aid of HPGe gamma spectrometry, inductively coupled plasma optical emission spectroscopy, DMA-80 Direct Mercury Analysis System, X-ray diffraction and statistical tools. At most of the locations, the radionuclides activity was higher than the world average activity concentration for the respective nuclei, 40K being the most abundant. From all the potentially toxic elements detected, Cr and Ni were usually observed on higher levels compared to their background values, indicating the probability of the detrimental biological effects. Thus, the present situation at the studied area might be a threat to the neighboring marine life.

Endemic sponge Lubomirskia baikalensis as a bioindicator of chemical elements pollution in Lake Baikal.

To evaluate the prospects of using Baikal endemic sponges as bioindicators of chemical elements pollution, the elemental composition of sponges, water and substrate samples, collected in two areas with different levels of anthropogenic loading of the Baikal Lake, was determined using two analytical techniques. The content of Cl, Ca, V, Zn, As, Se, Ba, Cd, and Cu in the sponges collected in Listvennichny Bay was significantly higher than in Bolshye Koty Bay. The values of the pollution indices point at the slight to moderate pollution of the substrates. According to the bioaccumulation factor values, sponges accumulate mainly Cd, Cu and Br from the substrate, and the main part of the elements from water. The distribution of elements longwise the sponges and their intraspecific variation were evaluated. It was shown that Lubomirskia baikalensis sponges were suitable bioindicators to assess the pollution of Lake Baikal.

Moss Biomonitoring of Atmospheric Trace Element Pollution in the Republic of Moldova.

The moss biomonitoring technique was used for the assessment of air pollution in the Republic of Moldova, in the framework of the UNECE ICP Vegetation Programme. The content of 11 chemical elements (Al, V, Cr, Fe, Ni, Zn, As, Sb, Cd, Cu, and Pb) was determined by neutron activation analysis and atomic absorption spectrometry in samples collected in spring 2020. Distribution maps were built to identify the most polluted sites. The highest concentrations of elements in mosses were determined in the north-eastern, central, and western parts of the country. The main element associations were identified using factor analysis. Three factors were determined, of which one of mixed geogenic-anthropogenic origin and two of anthropogenic origin. A comparison of the data obtained in 2020 and 2015 showed a significant decrease in the concentrations of Cr, As, Sb, Cd, Pb, and Cu in 2020. The state of the environment was assessed using Contamination Factor and Pollution Load Index values, which characterized it as unpolluted to moderately polluted. Possible air pollution sources in the Republic of Moldova are resuspension of soil particles, agricultural practices, vehicles, industry, and thermal power plants.

Moss Biomonitoring of Atmospheric Pollution with Trace Elements in the Moscow Region, Russia.

For the first time, moss biomonitoring covering the territory of the entire Moscow region, without including Moscow, was performed in 2020. Moss Pleurozium schreberi collected at 156 sampling sites were analyzed using neutron activation analysis and atomic absorption spectrometry. A total of 34 elements were determined in moss samples. Obtained data were compared with the results of the moss surveys performed in the Vladimir and Yaroslavl regions in 2018 and with the data of moss surveys conducted in the Moscow region on a limited number of sampling sites in 2004 and 2014. The Moscow region showed to be more polluted than the Vladimir and Yaroslavl regions. In the the Moscow region, the decrease of the content of the main part of the elements over time was noted. Trace elements emission sources were identified and characterized using factor analysis. Contamination Factor, Pollution Load Index, and Ecological Risk were calculated to assess the level of the region contamination and elements effect on human health. In general, the Moscow region can be characterized as unpolluted to moderately polluted with a low ecological risk of human exposure. The cities satellites around Moscow were determined to experience particular environmental stress, even in the period of the COVID-19 restrictions.

Determination of Multi Elements in Tobacco Plant of Northeast India by Neutron Activation Analysis and Atomic Absorption Spectrometry.

Even when cultivated in uncontaminated soils, tobacco plant has higher propensity to extract and accumulate trace elements. The concentrations (mass fractions) of essential elements (K, Ca, Mg, Na, Cl, Mn, Fe, Cu, and Zn) and 28 non-essential elements in tobacco plant (leaves, stem, and root) of Northeast India and their respective soils were quantitatively measured. Hg mass fraction in all samples analyzed were found to be < 10 mg/kg. The heavy element mass fractions of tobacco are weakly correlated to different soil parameters. The bioconcentration factor values indicated that Cd (7) is selectively absorbed and translocated in the tobacco leaves compared to Zn (1.7), Cu (1.5), Ni (0.12), and Pb (0.1). Under acidic soil conditions, tobacco plant efficiently absorbed and translocated Cl- ion with great ease, whereas it may be a very low accumulator of rare-earth elements. The mass fractions of Mn, Cu, Sb, Cs, Rb, and Pb are very similar to the "reference plant," whereas significantly higher mass fractions of Al, Sc, Ti, Zr, Hf, Ta, Th, and U are present in the roots of tobacco plant relative to the "reference plant." Principal component analysis has revealed that Northeast Indian tobacco can be clearly differentiated from other varieties of tobaccos used in different countries because of their element profiles.

Accumulation of Potentially Toxic Elements in Mosses Collected in the Republic of Moldova.

For the second time, the moss biomonitoring technique was applied to evaluate the deposition of potentially toxic elements in the Republic of Moldova. The study was performed in the framework of the International Cooperative Program on Effects of Air Pollution on Natural Vegetation and Crops. Moss Hypnum cupressiforme Hedw. samples were collected in May 2020 from 41 sampling sites distributed over the entire territory of the country. The mass fractions of 35 elements (Na, Mg, Al, Cl, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Br, Se, Rb, Sr, Sb, Cs, Ba, Cd, La, Ce, Sm, Eu, Tb, Hf, Ta, Th, Pb, and U) were determined using neutron activation analysis and atomic absorption spectrometry. Comparing with 2015/2016 moss survey data, significant differences in the mass fractions of Cr, As, Se, Br, Sr, Sb, Cd, Pb, and Cu were found. Main air pollution sources (natural processes, transport, industry, agriculture, mining) were identified and characterized using factor and correlation analyses. GIS maps were built to point out the zones with the highest element mass fractions and to relate this to the known sources of contamination. Contamination factor, geo-accumulation index, pollution load index, and potential ecological risk index were calculated to assess the air pollution levels in the country. According to the calculated values, Moldova can be characterized as unpolluted to moderately polluted, with low potential ecological risk related to the degree of atmospheric deposition of potentially toxic elements. The cities of Chisinau and Balti were determined to experience particular environmental stress and are considered moderately polluted.